Key lab12/20/2023 ![]() It is therefore a matter of finding the optimum balance of resolution and depth of field depending on the size and shape of the object’s features. Particularly at low magnifications, the depth of field can be significantly increased by stopping down, i.e., reducing the numerical aperture. įor the best possible visualization of an object, the proper adjustment of a modern microscope’s settings can produce an optimum balance between depth of field and resolution. The depth of field is determined by the correlation between numerical aperture, resolution, and magnification. ![]() The field number means that at a 1x combined magnification of the objective and zoom optics, the object field observed through the eyepieces is 23 mm in diameter. For example, eyepieces with a 10x magnification have a field number of 23. The diameter of the object field depends upon the total magnification. ![]() When looking into the eyepieces, a circular area called the object field becomes visible. Influence of magnification on the object field ![]() In general, values for M O are between 0.32x and 2x, for z between 0.63x and 16x, and for M E between 10x and 40x. M E is the magnification of the eyepieces. M O is the magnification of the objective (1x for the case of a Greenough System with no supplementary lens) M TOT VIS is the total magnification (VIS stands for "visual") The formula for total magnification is: M TOT VIS = M O x z x M E, where: ![]() To find out the magnification of the object observed via the eyepieces, the magnification factors of the objective, zoom optics, and eyepieces have to be multiplied. The eyepieces also have a constant magnification value. The zoom optics of the instrument allow the magnification to be changed over the zoom factor range. The objective has a fixed magnification value. The total magnification of a stereo microscope is the combined magnifying power of the objective lens, zoom optics, and eyepieces. ![]()
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